How do you identify phosphate solubilizing bacteria?
How do you identify phosphate solubilizing bacteria?
For P-solubilizing ability determination, the isolates were screened by culturing at 28°C on the media supplemented either lecithin (Po culture medium) or Ca3(PO4)2 (Pi culture medium). When the colonies appeared in 4–5 days, those causing a clear phosphate-solubilizing zone were selected out for further calculation.
Which medium is used for screening of phosphate solubilizing bacteria?
Pikovskaya (PVK) agar
Phosphate solubilizing microorganisms are routinely screened by a plate assay method using Pikovskaya (PVK) agar [5].
Which phosphate is solubilizing fungi?
The isolated phosphate solubilizing fungi belonged to genera of Aspergillus (55.69%), Penicillium spp. (23.35%), and Fusarium (9.58%).
Which bacteria is phosphate solubilizing?
Pseudomonas, Bacillus, Micrococcus, Aspergillus, Fusarium, etc. are the major phosphate solubilizing bacteria (PSB) in soil.
What is PSB culture?
Phosphate solubilizing bacteria (PSB) are beneficial bacteria capable of solubilizing inorganic phosphorus from insoluble compounds. P-solubilization ability of rhizosphere microorganisms is considered to be one of the most important traits associated with plant phosphate nutrition.
How do you isolate phosphate solubilizing bacteria?
Pikovskaya’s Agar Medium, is a selective medium for isolating PSB Species. The inoculated plates were incubated aerobically at 32°C for 9 to 10 days. After incubation, the colonies showing clear zone of phosphate solubilization were counted and expressed as colony forming unit (cfu) per gram of soil.
Which of the following media is used to isolate phosphate solubilizing bacteria?
Which of the following media is used to isolate phosphate solubilizing bacterial Species? Clarification: Pikovskaya’s Agar is a very special type agar that is used to isolate phosphate solubilizing bacterial species. The isolates can be isolated on Pikovskaya’s Agar after few hours-days of incubation.
How do you calculate phosphate solubilizing index?
A clear zone around a growing colony indicated phosphate solubilization and was measured as phosphate solubilization index (SI). SI was calculated as the ratio of the total diameter (colony + halo zone) to the colony diameter [18].
How do you isolate fungus in soil?
Techniques used for isolation of fungi from soil include serial dilution agar plate, Warcup soil plate, syringe inoculation, immersion tube method, screened immersion plates, plate profile, hyphal isolation, soil washing, partial presterlization, soil sieving, floatation, baiting etc.
Is a PSM fungi?
Phosphorus Solubilizing Microorganisms (PSM) A large number of microbial organisms including bacteria, fungi, actinomycetes, and algae exhibit P solubilization and mineralization ability.
Where are phosphate solubilizing bacteria found?
Phosphorus solubilizing microbes especially PSB are widely distributed in soils, freshwater, seawater, and sediments (Liu et al., 2014, 2017; Zhang et al., 2015), and responsible for the cycling of insoluble P to soluble PO43– ion.
What does phosphate solubilizing bacteria do?
Phosphate solubilizing microbes (PSMs) are a group of beneficial microorganisms capable of hydrolyzing organic and inorganic insoluble phosphorus compounds to soluble P form that can easily be assimilated by plants.