What is MEF feeder layer?
What is MEF feeder layer?
Embryonic Fibroblast (MEF) feeder cells are used for the maintenance of mouse or human ES cells in the undifferentiated state. The cells must be mitotically inactivated prior to the addition of ES cells, such as treatment with mitomycin C (2-4 hr, 10 µg/mL). Quality Control.
What is a feeder cell?
Basically, feeder cells consist in a layer of cells unable to divide, which provides extracellular secretions to help another cell to proliferate. It differs from a coculture system because only one cell type is capable to proliferate.
Why MEFs is treated with mitomycin?
Exposure to Mitomycin C at 10 Rg/mL for 2-3 hours is sufficient to mitotically inactivate MEFs. Inactivated MEFs are counted and plated on tissue culture vessels to give the desired MEF concentration for the culture of the ESC line. Mitomycin C inhibits DNA synthesis and nuclear division.
Is Mitomycin considered chemotherapy?
Mitomycin is a chemotherapy drug used to treat different cancers including breast, bladder, stomach, pancreatic, anal and lung cancers. This information is about mitomycin given into a vein. We have other information about: mitomycin and 5FU when it is given along with radiotherapy (called chemoradiation).
How long is Mitomycin treatment?
Your treatment
| Intravesical Mitomycin | ||
|---|---|---|
| This treatment cycle is repeated every 7 days. You will have 6 cycles. | ||
| Day | Treatment | How long it takes |
| 1 | Mitomycin (my-tow-my-sin) | About 2 to 3 hours |
What is STO cell line?
Abstract. STO feeder cells, a line established from mouse SIM embryonic fibroblasts, have been frequently used for establishing embryonic stem cells and maintaining them in an undifferentiated state.
What is MEF cells?
Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmission, MEFs will senesce and finally die off.
What are types of feeder layers?
Richards et al. compared the growth of two hESC lines on three different human feeder layers (fetal muscle, fetal skin, and adult fallopian tubal epithelial cells) and on feeder-free matrices with the conditioned medium prepared from the three human feeders and from MEF.
What does mitomycin C do to cells?
Overview. Mitomycin C is an antibiotic which acts as a double-stranded DNA alkylating agent. It covalently crosslinks DNA, inhibiting DNA synthesis and cell proliferation.
What are fibroblast feeder cells?
Feeder cells support the growth of cells in culture by contributing an undefined and complex mixture of extracellular matrix (ECM) components, and growth factors. Usually, feeder cells used for the co-culture of embryonic stem cells (ESCs) are fibroblasts, and mitotically inactivated so that they remain viable.