What is a substrate solution?

What is a substrate solution?

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets.

What is TMB substrate in ELISA?

Description. 3,3’5,5′-Tetramethylbenzidine (TMB) is a safe-sensitive substrate used for the detection of Horseradish Peroxidase (HRP) activity. It yields a blue colour (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid.

Why is alkaline phosphatase used in ELISA?

Most ELISA tests have used alkaline phosphatase conjugated antibodies for the detection system as it used the substrate p-nitrophenyl phosphate which produces a water-soluble yellow reaction product.

What is called substrate?

1 : substratum. 2 : the base on which an organism lives the soil is the substrate of most seed plants. 3 : a substance acted upon (as by an enzyme)

What is substrate and reagent?

The substrate is a molecule which is used as a reactant in the reaction. The substrate is a molecule over which the enzyme acts. The action of enzyme causes the substrate to transform it into product. The reagent is a chemical molecule which can be a single compound, or mixture of compounds.

What is TMB substrate?

TMB (3,3′,5,5′-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP), a common label conjugated to antibodies for applications such as immunohistochemistry, western blotting, and ELISA. The combination of HRP and hydrogen peroxide causes the oxidation of TMB, resulting in the formation of a blue color.

What are substrates used for?

In atomic layer deposition, the substrate acts as an initial surface on which reagents can combine to precisely build up chemical structures. A wide variety of substrates are used depending on the reaction of interest, but they frequently bind the reagents with some affinity to allow sticking to the substrate.