How do you do Boc deprotection?
How do you do Boc deprotection?
2-DEPROTECTION The deprotection of a BOC-protected amine is a simple carbamate hydrolysis in acidic conditions. The starting material is dissolved in water or organic solvent, such as toluene, dichloromethane, or ethyl acetate. Concentrated hydrochloric acid, or trifluoroacetic acid (TFA) are the acids of choice.
What is Cbz in organic chemistry?
Benzyl chloroformate, also known as benzyl chlorocarbonate or Z-chloride, is the benzyl ester of chloroformic acid. It can be also described as the chloride of the benzyloxycarbonyl (Cbz or Z) group. In its pure form it is a water-sensitive oily colorless liquid, although impure samples usually appear yellow.
How do I uninstall Cbz?
Cbz is removed by hydrogenation, -Boc removed with acid, and -Fmoc with base.
How is the BOC group of an amino acid derivative removed?
Removal of the BOC in amino acids can be accomplished with strong acids such as trifluoroacetic acid in dichloromethane, or with HCl in methanol. A complication may be the tendency of the t-butyl cation intermediate to alkylate other nucleophiles; scavengers such as anisole or thioanisole may be used.
How much TFA do I need for Boc deprotection?
20 mL
Boc-L-Glu(Z)-Me (2.11 g, 6.00 mmol) was reacted following the general procedure for the deprotection of the Boc-group. TFA (20 mL), CH2Cl2 (80 mL). The product was used without further purification and analysis to avoid diketopiperazine-formation.
What is a deprotection reaction?
Definition: A reaction in which a protecting group is modified by converting it into a functional group.
What is full form of Cbz?
There are various course types in Bachelor of Science, and one of them is BSc CBZ – chemistry, botany, and zoology.
What is BSc in Cbz?
Bachelor of Science in Chemistry, Botany, Zoology – BSc. CBZ.
How do I uninstall TFA after Boc deprotection?
TFA can be removed using rotary evaporator. Add Methanol (5~6 times) and remove methanol by rotary evaporator. Most of the TFA is removed using this procedure and traces can be removed using vacuum.