What is a fluorescence plate reader?
What is a fluorescence plate reader?
A fluorescence plate reader, (longform: fluorescence microplate reader; shortform: fluorescence reader) also called spectrofluorometer or fluorometer, is a piece of equipment capable of detecting and quantifying the light photons emitted by a fluorescent sample present in a microplate upon excitation with light at a …
How is fluorescence measured in a plate reader?
An Introduction to Plate Readers Luminescence and fluorescence signals are detected and quantified using luminometers and fluorometers, respectively, and colorimetric and absorbance measurements are detected by light absorption at specific wavelengths.
Which is best microplate reader?
Top List: Top Microplate Readers as Reviewed by You
- CLARIOstar® (BMG LABTECH)
- SpectraMax® M5e Microplate Reader (Molecular Devices)
- Cytation 3 Cell Imaging Multi-Mode Reader (BioTek Instruments Inc.)
- Mithras LB 940 Multimode Microplate Reader (BERTHOLD TECHNOLOGIES GmbH & Co.
Can I use clear plates for fluorescence?
Transparent plates should not be used with fluorescence as reflections or unspecific signals can significantly impair sensitivity.
What does a microplate reader do?
A microplate reader is a laboratory instrument that is used to measure chemical, biological or physical reactions, properties and analytes within the well of a microplate. A microplate consists of small wells in which separated reactions take place.
How do I choose a plate reader?
Primary considerations when choosing a microplate reader are throughput and flexibility. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile.
What are microplate readers used for?
What plate should I use for luminescence?
White plates
White plates are used for luminescence and black plates are used for fluorescence. Plates with clear bottoms are used for bottom reading, and plates with white or black bottoms are used for top reading.