Is IF2 a GTPase?
Is IF2 a GTPase?
IF2 is a GTPase that functions to position the initiator tRNA within the 30S ribosomal initiation complex (30S IC) and promotes its joining with the 50S ribosomal subunit to form a 70S ribosome.
What happens in initiation of protein synthesis?
The initiation of protein synthesis is the process that results in bringing together an 80S ribosome with a messenger RNA (mRNA) and initiator methionyl-transfer RNA (Met-tRNAi).
Does translation termination require GTP?
GTP hydrolysis catalyzed in the ribosome by a complex of two polypeptide release factors, eRF1 and eRF3, is required for fast and efficient termination of translation in eukaryotes.
Is IF2 polar or nonpolar?
IF2- is nonpolar in nature because its molecular shape is linear that means dipole that is induced because of the difference in electronegativity value of iodine and fluorine atoms are strictly opposite in direction to each other.
What happens in initiation elongation and termination?
During elongation, amino acids are continually added to the line, forming a long chain bound together by peptide bonds. Once a stop codon reaches the ribosome, translation stops, or terminates. At termination, the polypeptide is freed from the ribosome, and tRNAs stop bringing the amino acids in.
What are IF1 IF2 and IF3?
Bacterial translation initiation is a multistep process, which requires initiation factor 1 (IF1), IF2 and IF3. In the first phase of initiation, IF1, IF2 (a GTP-binding protein), IF3, mRNA and the initiator fMet-tRNAfMet bind to the 30S ribosomal subunit, forming the 30S initiation complex (30S IC).
What is the function of IF1?
IF1 associates with the 30S ribosomal subunit in the A site and prevents an aminoacyl-tRNA from entering. It modulates IF2 binding to the ribosome by increasing its affinity. It may also prevent the 50S subunit from binding, stopping the formation of the 70S subunit.
What happens in termination of transcription?
Transcription termination occurs when a transcribing RNA polymerase releases the DNA template and the nascent RNA. Termination is required for preventing the inappropriate transcription of downstream genes, and for recycling of the polymerase.