What enzyme catalyzes the Anaplerotic reaction of oxaloacetate?
What enzyme catalyzes the Anaplerotic reaction of oxaloacetate?
Pyruvate carboxylase (PC) is a biotin- and ATP-dependent mitochondrial enzyme that catalyzes the anaplerotic carboxylation of pyruvate to oxaloacetate, a substrate for gluconeogenesis. This is the point at which not only pyruvate, but also lactate and alanine, enter the gluconeogenic pathway.
How are amino acids converted to oxaloacetate?
Aspartate is converted into oxaloacetate by a reaction catalyzed by the enzyme aspartate aminotransferase, which transfers an amino group from aspartate to 2-oxoglutarate to produce glutamate and oxaloacetate.
Which of the following enzymes catalyzes an Anaplerotic reaction for the citric acid cycle?
Pyruvate carboxylase, which generates oxalacetate directly in the mitochondria, is the major anaplerotic enzyme. Conversely, 4- and 5-carbon intermediates enter the TCA cycle during the catabolism of amino acids.
What is an anaplerotic enzyme?
Anaplerotic enzyme reactions are those which replenish tricarboxylic acid intermediates that are withdrawn for the synthesis of biomass. In this study, we examined recombinant protein production in Escherichia coli containing activity in an additional anaplerotic enzyme, pyruvate carboxylase.
Which amino acid is converted into oxaloacetate in a transamination reaction?
Glutamate’s amino group, in turn, is transferred to oxaloacetate in a second transamination reaction yielding aspartate.
How is oxaloacetate formed?
Carboxylation of pyruvate produces oxaloacetate (OAA). This is an energy-requiring reaction that uses adenosine triphosphate (ATP).
What enzyme replenishes oxaloacetate?
pyruvate carboxylase
One important way by which human cells and other mammalian cells replenish the intermediates is by replenishing oxaloacetate; this is done by carboxylating pyruvate into oxaloacetate via a process that is catalyzed by an enzyme called pyruvate carboxylase.