What is anti tubulin antibody?
What is anti tubulin antibody?
Product Description. Rabbit anti Porcine tubulin antibody recognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Why is tubulin used in Western blots?
Beta-Tubulin, is usually used as loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What does alpha tubulin do?
Highly conserved α- and β-tubulin heterodimers assemble into dynamic microtubules and perform multiple important cellular functions such as structural support, pathway for transport and force generation in cell division.
What are tubulin binding agents?
An antimitotic tubulin-binding agent with potential antineoplastic activity. Tubulin-binding agent SSR97225 binds to tubulin, arresting the cell cycle at the G2/M checkpoint and preventing mitosis.
Is tubulin present in all cells?
In eukaryotes, there are six members of the tubulin superfamily, although not all are present in all species.
Is beta tubulin a good loading control?
Nonetheless, β-actin and β-tubulin offer certain advantages as loading controls: they are highly conserved, display high expression level, and exhibit stability under most experimental conditions.
Where is alpha tubulin located?
Most cells produce α-tubulin, but the protein is found in highest amounts in the developing brain. During brain development, α-tubulin partners with β-tubulin to form microtubules that move nerve cells (neurons) to their proper location (neuronal migration). Microtubules form scaffolding within the cell.
Where is alpha tubulin found in a cell?
Overview. Alpha and beta tubulins are globular proteins and exist included in five main forms/subunits as well as delta, gamma, and epsilon. As building blocks of various structures, tubulins belong to a group of proteins found in the cell cytoplasm in high amounts.
Where are alpha and beta tubulin located?
cell cytoplasm
Overview. Alpha and beta tubulins are globular proteins and exist included in five main forms/subunits as well as delta, gamma, and epsilon. As building blocks of various structures, tubulins belong to a group of proteins found in the cell cytoplasm in high amounts.