What is meant by Michaelis-Menten constant?
What is meant by Michaelis-Menten constant?
The Michaelis constant (KM), defined as the concentration of substrate that is transported at half the maximal velocity (Vmax) of transport, is a measure of the affinity of the transporter for its substrate.
What does Michaelis-Menten constant depend on?
is dependent on both the identity of enzyme and that of the substrate, as well as conditions such as temperature and pH.
What is the value of Michaelis constant?
The Michaelis constant Km is equal to the reactant concentration at which rA=vmax/2. Km is independent of enzyme concentration but varies from one enzyme to another and with different substrates for the same enzyme. Values of Km for some enzyme–substrate systems are listed in Table 12.3.
What is the unit of Michaelis constant?
Michaelis constant is the substrate concentration at which the reaction velocity is equal to half the maximal velocity of the reaction. The units of Km are M, concentration. Km indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.
What is Michaelis constant in biology?
Definition of Michaelis constant : a constant that is a measure of the kinetics of an enzyme reaction and that is equivalent to the concentration of substrate at which the reaction takes place at one half its maximum rate.
What is Significance of Km?
Significance of Km and Vmax 1) Km value is used as a measure of an enzyme’s affinity for its substrate. The lower the Km value the higher the enzyme’s affinity for the substrate and vice versa. 2) Km value also provides an idea of the strength of binding of the substrate to the enzyme molecule.
Why is Michaelis constant important?
Significance of Michaelis-Menten Constant: (i) By knowing the Km value of a particular enzyme-substrate system, one can predict whether the cell needs more enzymes or more substrate to speed up the enzymatic reaction.
What is the function of Michaelis Menten constant and its significance?
Michaelis constant is a reflection of the affinity of enzyme for its substrate and is characteristic of a particular enzyme-substrate system. The smaller the value of Km, the more strongly the enzyme binds the substrate.
What is Km value in Michaelis-Menten?
The Michaelis constant (KM) is defined as the substrate concentration at which the reaction rate is half of its maximal value (or in other words it defines the substrate concentration at which half of the active sites are occupied).